Table 5 Hydrogen production from microalgae pretreated by biological methods
From: Fermentative hydrogen production using pretreated microalgal biomass as feedstock
Treatment methods | Substrate | Substrate concentration (g/L TS) | Inoculum | Operational conditions | Hydrogen yield (mL H2/g VS) | Comments | References |
|---|---|---|---|---|---|---|---|
Biological: Onozuka R-10 enzyme | Chlorella vulgaris | 10 | Anaerobic sludge | pH = 7.5, 60 °C; batch | 39 | Onozuka R-10 enzyme treatment increased hydrogen production from Chlorella vulgaris biomass from 19 to 39 mL/g VS | [15] |
Biological: macerozyme R-10 enzyme | Chlorella vulgaris | 10 | Anaerobic sludge | pH = 7.5, 60 °C; batch | 62 | Macerozyme R-10 enzyme showed better effect on hydrogen production from Chlorella vulgaris biomass than Onozuka R-10 enzyme | [15] |
Biological: Onozuka R-10 enzyme + macerozyme R-10 enzyme | Chlorella vulgaris | 10 | Anaerobic sludge | pH = 7.5, 60 °C; batch | 135 | Combination of Onozuka R-10 enzyme and macerozyme R-10 enzyme treatment resulted in significant increase in hydrogen yield from Chlorella vulgaris biomass than single enzyme treatment | [15] |
Biological: microbial consortium TC60, 60 °C, 10 days | Chlorella vulgaris | 0.14a | TC60 from compost | pH = 7.0, 60 °C; batch | 11 | Chlorella biomass showed recalcitrance to anaerobic digestion by TC60, and hydrogen was produced by satellite heterotrophs from C. vulgaris | [27] |
Microbial consortium TC60, 60 °C, 10 days | Dunaliella tertiolecta | 0.094a | TC60 from compost | pH = 7.0, 60 °C; batch | 13 | Hydrogen yields increased at least 10% after biological treatment process. Digestion of Dunaliella tertiolecta provided additional nutrients for cellulolytic activity | [27] |