Fig. 4
From: Improved fatty aldehyde and wax ester production by overexpression of fatty acyl-CoA reductases
Thin layer chromatography analysis demonstrating the effect of the FAR overexpression on the WE production. The S1 strain harboring different FARs under an IPTG-inducible promoter were cultivated at 30 °C for 48 h with or without IPTG, the lipids extracted and analyzed with thin layer chromatography. The control strain iluxAB has the wild type expression of Acr1. Jojoba oil (1 mg/ml) was used as a wax ester standard. Equal volume of each culture were used for the extraction. Two replicates were analyzed and a representative picture is shown. The clarity of the picture was enhanced by adjusting the contrast