Establishment of a yeast-based VLP platform for antigen presentation

Table 4 Summary of analytical results on target protein production and characterization

Designation of analyzed strains	Fusion protein co-produced with dS	N-Glycosylation of the fusion protein detected	Identity of foreign antigen confirmed	Chimeric VLP formation
D#113	E2BVDV344-dS	Yes	Yes	Yes
D#106	E2BVDV196-dS	Yes	Yes	Yes
D#117	dS-E2BVDV196	No	No	Yes
D#53	E2CSFV337-dS	Yes	Yes	Yes
D#73	E2CSFV184-dS	Yes	Yes	Yes
D#79	E2CSFV102-dS	No	Yes	Yes
M#22-8	FeLVp45-dS	Yes	NE	Yes
M#4-5	FeLVp45-dS and FeLVp15E-dS	NE	NE	Yes^a
T#3-3	EDIIIWNV-dS	NE	Yes	Yes
Assay applied	Anti-dS Western blot	Protein deglycosylation assay	Western blot^b	Ultracentrifugation

NE not examined
^aFormation of chimeric VLP composed of dS, FeLVp45-dS and FeLVp15E-dS
^bApplying primary antibodies specific for the respective foreign antigen as indicated in Table 1

ISSN: 1475-2859