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Table 2 Summary of fusion proteins constructed and recombinantly produced in H. polymorpha

From: Establishment of a yeast-based VLP platform for antigen presentation

Viral source protein (Genbank accession number) aa used for fusion protein (number of predicted N-glycosylation sitesa) Fusion protein designation C- or N-terminally fused to dS Number of aab MW [kDa] Signal sequence (CL) or start methionine (M) included Genbank accession number for the fusion protein coding gene
BVDV E2 (AEV54362.1) 1–344 (4) E2BVDV344-dS N 511 57.5 CL MG280712
1–196 (2) E2BVDV196-dS N 363 40.3 CL MG280713
1–196 (2) dS-E2BVDV196 C 363 40.3 M MG280714
CSFV E2 (AAT85717.1) 1–337 (5) E2CSFV337-dS N 504 56.1 CL MG280715
1–184 (2) E2CSFV184-dS N 351 38.7 CL MG280716
1–102 (0) E2CSFV102-dS N 270 29.5 M MF510123
FeLV env (AAA43051.1) 1–412 (9) FeLVp45-dS N 580 63.7 M MG280717
1–132c (0) FeLVp15E-dS N 326 35.8 M MG280718
WNV E (ADI33161.1) 293–454 (0) EDIIIWNV-dS N 330 35.3 M MG280719
  1. aPredicted by NetNGlyc 1.0 [76]
  2. b167 aa of the dS included, mature protein
  3. cIncluding the single aa exchange E82R [77]