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Table 2 Summary of fusion proteins constructed and recombinantly produced in H. polymorpha

From: Establishment of a yeast-based VLP platform for antigen presentation

Viral source protein (Genbank accession number)

aa used for fusion protein (number of predicted N-glycosylation sitesa)

Fusion protein designation

C- or N-terminally fused to dS

Number of aab

MW [kDa]

Signal sequence (CL) or start methionine (M) included

Genbank accession number for the fusion protein coding gene

BVDV E2 (AEV54362.1)

1–344 (4)

E2BVDV344-dS

N

511

57.5

CL

MG280712

1–196 (2)

E2BVDV196-dS

N

363

40.3

CL

MG280713

1–196 (2)

dS-E2BVDV196

C

363

40.3

M

MG280714

CSFV E2 (AAT85717.1)

1–337 (5)

E2CSFV337-dS

N

504

56.1

CL

MG280715

1–184 (2)

E2CSFV184-dS

N

351

38.7

CL

MG280716

1–102 (0)

E2CSFV102-dS

N

270

29.5

M

MF510123

FeLV env (AAA43051.1)

1–412 (9)

FeLVp45-dS

N

580

63.7

M

MG280717

1–132c (0)

FeLVp15E-dS

N

326

35.8

M

MG280718

WNV E (ADI33161.1)

293–454 (0)

EDIIIWNV-dS

N

330

35.3

M

MG280719

  1. aPredicted by NetNGlyc 1.0 [76]
  2. b167 aa of the dS included, mature protein
  3. cIncluding the single aa exchange E82R [77]