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Fig. 3 | Microbial Cell Factories

Fig. 3

From: CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus

Fig. 3

Activation effects of the epothilone gene cluster by the ω subunit of RNA polymerase. a The yields of epothilones A and B and their summation in mutants with the p41sg plasmids containing different spacers (YL1612, 95-nt TSS1/coding; YL1613, 93-nt TSS1/noncoding; YL1614, 120-nt TSS2/noncoding; YL1615, 103-nt TSS1/coding; YL1616, 106-nt TSS2/coding) and the ancestral strain M. xanthus ZE9. b RT-qPCR analysis of expression levels of the seven genes for the biosynthesis of epothilones in different mutants at 48 h of incubation. The expressions of the seven genes of the gene cluster for the biosynthesis of epothilones in M. xanthus ZE9 were each set as 1, and the expressions of the seven genes of mutant strains were shown as the relative expressions to that of their corresponding genes in ZE9. The error bars represent the standard deviation of three independent experiments. For statistical analysis between the ancestral strain and mutant strains, the signals of ** and * mean p < 0.01 and p < 0.05, respectively. c RT-qPCR analysis of the expression levels of the seven genes for the biosynthesis of epothilones of M. xanthus ZE9 at 48 h of incubation. The expression of epoA gene was set as 1, and the expressions of the other six genes in ZE9 were the relative expressions to that of epoA

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