Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Table 4 Effects of various organic solvents and detergents on the activity of DMWf18-543 and DMWf18-558

Solvents/5%	Relative activity (%)		Solvents/15%	Relative activity (%)		Detergents/1%	Relative activity (%)
Solvents/5%	DMWf18-543	DMWf18-558	Solvents/15%	DMWf18-543	DMWf18-558	Detergents/1%	DMWf18-543	DMWf18-558
Control	100.0 ± 0.7	100.0 ± 1.0	Control	100.0 ± 1.2	100.0 ± 2.5	Control	100 ± 1.1	100 ± 0.3
Acetone*	57.8 ± 0.1	20.8 ± 0.4	Acetone	0	0	SDS	0	0
Acetonitrile*	53.0 ± 0.1	33.8 ± 0.3	Acetonitrile	0	0	TritonX-100*	45.1 ± 0.9	9.5 ± 0.1
Alcohol*	56.4 ± 0.4	31.2 ± 0.3	Alcohol*	0	4.0 ± 0.7	Tween 20*	66.6 ± 0.5	28.6 ± 0.9
DMF*	58.6 ± 0.7	14.0 ± 0.6	DMF	0	0	Tween 80*	56.1 ± 0.6	11.3 ± 0.6
DMSO*	90.4 ± 0.1	52.5 ± 2.3	DMSO*	38.0 ± 0.1	23.8 ± 0.3
Glycerol	98.0 ± 0.8	98.2 ± 2.4	Glycerol*	85.2 ± 0.7	103.0 ± 1.9
Isopropanol*	67.1 ± 0.6	20.7 ± 0.1	Isopropanol	0	0
Methanol*	81.0 ± 0.3	75.6 ± 1.9	Methanol*	5.5 ± 0.1	0

The activity observed without an organic solvent or detergent was taken as 100%. * p < 0.05, representing a significant difference between DMWf18-543 and DMWf18-558 (Student’s t test)

ISSN: 1475-2859