Skip to main content

Table 4 Effects of various organic solvents and detergents on the activity of DMWf18-543 and DMWf18-558

From: Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Solvents/5% Relative activity (%) Solvents/15% Relative activity (%) Detergents/1% Relative activity (%)
DMWf18-543 DMWf18-558 DMWf18-543 DMWf18-558 DMWf18-543 DMWf18-558
Control 100.0 ± 0.7 100.0 ± 1.0 Control 100.0 ± 1.2 100.0 ± 2.5 Control 100 ± 1.1 100 ± 0.3
Acetone* 57.8 ± 0.1 20.8 ± 0.4 Acetone 0 0 SDS 0 0
Acetonitrile* 53.0 ± 0.1 33.8 ± 0.3 Acetonitrile 0 0 TritonX-100* 45.1 ± 0.9 9.5 ± 0.1
Alcohol* 56.4 ± 0.4 31.2 ± 0.3 Alcohol* 0 4.0 ± 0.7 Tween 20* 66.6 ± 0.5 28.6 ± 0.9
DMF* 58.6 ± 0.7 14.0 ± 0.6 DMF 0 0 Tween 80* 56.1 ± 0.6 11.3 ± 0.6
DMSO* 90.4 ± 0.1 52.5 ± 2.3 DMSO* 38.0 ± 0.1 23.8 ± 0.3    
Glycerol 98.0 ± 0.8 98.2 ± 2.4 Glycerol* 85.2 ± 0.7 103.0 ± 1.9    
Isopropanol* 67.1 ± 0.6 20.7 ± 0.1 Isopropanol 0 0    
Methanol* 81.0 ± 0.3 75.6 ± 1.9 Methanol* 5.5 ± 0.1 0    
  1. The activity observed without an organic solvent or detergent was taken as 100%. * p < 0.05, representing a significant difference between DMWf18-543 and DMWf18-558 (Student’s t test)