Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Table 3 Effects of various metal ions and chelation on the activity of DMWf18-543 and DMWf18-558

Metals and chelating agent/10 mM	Relative activity (%)
Metals and chelating agent/10 mM	DMWf18-543	DMWf18-558
Control	100.0 ± 0.5	100.0 ± 2.0
Ba*	88.0 ± 0.2	97.9 ± 0.3
Ca*	78.1 ± 0.8	81.8 ± 2.0
Co*	30.9 ± 0.1	15.6 ± 1.3
Cu	0	0
Mg	97.8 ± 0.4	92.6 ± 5.9
Mn*	38.4 ± 0.4	35.2 ± 1.1
Ni*	22.4 ± 1.8	0
Sr*	80.4 ± 0.6	99.8 ± 0.9
Zn*	21.2 ± 0.4	15.9 ± 3.1
EDTA	117.7 ± 0.5	124.9 ± 4.3

The activity observed without metal ion or chelation was taken as 100%. * p < 0.05, representing a significant difference between DMWf18-543 and DMWf18-558 (Student’s t test)

ISSN: 1475-2859