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Table 3 Effects of various metal ions and chelation on the activity of DMWf18-543 and DMWf18-558

From: Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Metals and chelating agent/10 mM Relative activity (%)
DMWf18-543 DMWf18-558
Control 100.0 ± 0.5 100.0 ± 2.0
Ba* 88.0 ± 0.2 97.9 ± 0.3
Ca* 78.1 ± 0.8 81.8 ± 2.0
Co* 30.9 ± 0.1 15.6 ± 1.3
Cu 0 0
Mg 97.8 ± 0.4 92.6 ± 5.9
Mn* 38.4 ± 0.4 35.2 ± 1.1
Ni* 22.4 ± 1.8 0
Sr* 80.4 ± 0.6 99.8 ± 0.9
Zn* 21.2 ± 0.4 15.9 ± 3.1
EDTA 117.7 ± 0.5 124.9 ± 4.3
  1. The activity observed without metal ion or chelation was taken as 100%. * p < 0.05, representing a significant difference between DMWf18-543 and DMWf18-558 (Student’s t test)