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Table 2 Kinetic parameters of DMWf18-543, DMWf18-558 and the mutant protein

From: Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Enzyme pH Vmax (μmol/mg/min) Km (mM) kcat (s−1) kcat/Km (mM−1/s−1)
DMWf18-543 p-NP butyrate 5957 ± 112 0.33 ± 0.02 3209 ± 60 9610
p-NP hexanoate 108 ± 3 0.54 ± 0.04 58 ± 1 107
DMWf18-558 p-NP butyrate 5523 ± 228 0.60 ± 0.06 2957 ± 122 4854
p-NP hexanoate 5240 ± 145 0.076 ± 0.009 2805 ± 78 37,120
DMWf18-558-A199L p-NP butyrate 2101 ± 32 0.16 ± 0.01 1126 ± 17 7239
p-NP hexanoate 909 ± 14 0.034 ± 0.003 487 ± 8 14,542