Two novel deep-sea sediment metagenome-derived esterases: residue 199 is the determinant of substrate specificity and preference

Table 2 Kinetic parameters of DMWf18-543, DMWf18-558 and the mutant protein

Enzyme	pH	Vmax (μmol/mg/min)	K_m (mM)	kcat (s⁻¹)	kcat/K_m (mM⁻¹/s⁻¹)
DMWf18-543	p-NP butyrate	5957 ± 112	0.33 ± 0.02	3209 ± 60	9610
DMWf18-543	p-NP hexanoate	108 ± 3	0.54 ± 0.04	58 ± 1	107
DMWf18-558	p-NP butyrate	5523 ± 228	0.60 ± 0.06	2957 ± 122	4854
DMWf18-558	p-NP hexanoate	5240 ± 145	0.076 ± 0.009	2805 ± 78	37,120
DMWf18-558-A199L	p-NP butyrate	2101 ± 32	0.16 ± 0.01	1126 ± 17	7239
DMWf18-558-A199L	p-NP hexanoate	909 ± 14	0.034 ± 0.003	487 ± 8	14,542

ISSN: 1475-2859