Fig. 3

Characterization of DMWf18-543 and DMWf18-558. a Substrate specificity was determined using the p-NP esters, including p-NP acetate (C2), p-NP butyrate (C4), p-NP caprylate (C8), p-NP decanoate (C10), p-NP laurate (C12), p-NP myristate (C14), and p-NP palmitate (C16). All of the tests were performed at 35 °C and pH 7.5. b Effects of pH on the activity were determined in different buffers: 100 mM citrate buffer (pH 3.0–6.0), 100 mM phosphate buffer (pH 6.0–7.5), 100 mM tricine buffer (pH 7.5–9.0), and 50 mM CHES buffer (pH 9.0–10.0). All of the tests were performed at 35 °C using p-NP butyrate as the substrate. c Effects of temperature on the activity were determined at various temperatures at pH 7.5 using p-NP butyrate as the substrate. The highest activity was taken as 100%. Data are presented as the mean ± SD (n = 3)