Table 1 Strains and plasmids used in this study

Strains

 EGS1405

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB

[2]

 EGS1895

EGS1405 with pEG1675

[2]

 XW1003

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; ΔptsG

This study

 XW1004

XW1003 with pEG1675

This study

 XW1013

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up

This study

 XW1014

XW1013 with pEG1675

This study

 XW1018

XW1013 with pXW1678

This study

 XW1023

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up; ΔaraE

This study

 XW1024

XW1023 with pEG1675

This study

 XW1043

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up; ΔaraE; P_CP25-araB; ΔaraC; P_CP6-araF

This study

 XW1044

XW1043 with pXW1677

This study

 XW1053

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up; Δpgi

This study

 XW1054

XW1053 with pEG1675

This study

 XW1055

XW1053 with pXW1678

This study

 XW1063

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up; crp-RBS (TIR = 13)

This study

 XW1064

XW1063 with pEG1675

This study

 XW1073

E. coli DH1; ΔfadE; ΔackA-pta; ΔpoxB; P_CP6-xylF; P_CP25-xylA; xylA^up; crp-RBS (TIR = 13); Δpgi

This study

 XW1074

XW1073 with pEG1675

This study

 XW1075

XW1073 with pXW1678

This study

Plasmids

 pKD13

λ-Red recombineering plasmid

[19]

 pKD46

λ-Red recombineering plasmid

[19]

 pCP20

λ-Red recombineering plasmid

[19]

 pEG1675

Km^r, araC-P_BAD-fadR-co_fadD; P_trc-fadM; P_lacUV5-‘tesA-fadB-co_aco

[2]

 pXW1677

pEG1675 with araC deleted

This study

 pXW1678

Km^r, araC-P_BAD-fadR-co_fadD; P_tr-fadM-maeB; P_lacUV5-‘tesA-fadB-co_aco

This study