Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant genotype or description | Source or references |
|---|---|---|
Strains | ||
B. subtilis | ||
PKS11 | trpC2 pLS20cat | [15] |
GR138 | trpC2 pLS20cat pGR16B | [19] |
TMO310 | trpC2 aprE::(spc lacI Pspac-mazF) | [20] |
TMO311 | trpC2 aprE::(kan lacI Pspac-mazF) | [20] |
YNB001 | trpC2 comK::spc | This study |
YNB022 | trpC2 pLS20cat (kan lacI Pspac-mazF) | This study |
YNB026 | trpC2 pLS20catΔoriT | This study |
YNB031 | trpC2 pLS20catΔoriT pGR16B | This study |
YNB060 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) | This study |
YNB061 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) | This study |
YNB069 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) | This study |
YNB062 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) | This study |
YNB097 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) | This study |
YNB065 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) pLS20cat | This study |
YNB066 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) pLS20cat | This study |
YNB071 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) pLS20cat | This study |
YNB067 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) pLS20cat | This study |
YNB099 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) pLS20cat | This study |
YNB091 | trpc2 aprE::kan yhfM::(oriTLS20-F erm) pLS20catΔoriT | This study |
YNB095 | trpc2 aprE::kan yhfM::(oriTLS20-R erm) pLS20catΔoriT | This study |
YNB092 | trpc2 aprE::kan yhfK::(oriTLS20-F erm) pLS20catΔoriT | This study |
YNB094 | trpc2 aprE::kan yhfC::(oriTLS20-F erm) pLS20catΔoriT | This study |
YNB100 | trpc2 aprE::kan yhcT::(oriTLS20-F erm) pLS20catΔoriT | This study |
Plasmids | ||
pLS20cat | Conjugative plasmid pLS20 with a chloramphenicol resistance gene inserted in the unique Sall site | [18] |
pLS20catΔoriT | pLS20cat without oriTLS20 | This study |
pGR16B | Mobilizable plasmid containing oriTLS20 and erythromycin resistance gene | [19] |