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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer

Fig. 2

Mobilization efficiencies of the mobilizable plasmid, pGR16B, and the helper plasmids, pLS20cat and pLS20catΔoriT. Liquid cultures of the recipient strain YNB001 (comK::spc) and one of the donor strains: PKS11 (168 with pLS20cat), YNB026 (168 with pLS20catΔoriT), GR138 (168 with pLS20cat and pGR16B), and YNB031 (168 with pLS20catΔoriT and pGR16B), were mixed for conjugative transfer and spread on LB plates containing both chloramphenicol and spectinomycin (CS), both erythromycin and spectinomycin (ES), chloramphenicol, erythromycin, and spectinomycin (CES), and spectinomycin alone. Colonies were counted as CFUs to calculate mobilization efficiencies [CFU of transconjugants (colonies on the CS, ES, and CES plates)/CFU of total recipients (colonies on the spectinomycin plate) × 106 (ppm)]. Values are means with standard deviations from three independent experiments. ND not detected (< 0.01 ppm). NP not performed

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Microbial Cell Factories

ISSN: 1475-2859

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