Skip to main content

Table 1 Bacterial strains, and plasmids used in this study

From: Identification, synthesis and regulatory function of the N-acylated homoserine lactone signals produced by Pseudomonas chlororaphis HT66

Strain, plasmid Characteristics Reference or source
Escherichia coli strains
 E.coli DH5α supE44∆lacU169(ɸ80lacZ∆M15)recAhsdR17 recA1 endA1 gyrA96 thi-1 relA-1 Hanahan [45]
 E. coli S17 res- pro mod+ integrated copy of RP4, mob+, used for incorporating constructs in P. chlororaphis Hoffmann et al. [46]
 A. tumefaciens NTL4 (pZLR4) Biosensor strain for AHLs Cha et al. [28]
 C. violaceum CV026 Biosensor strain for AHLs McClean et al. [29]
 Pythium ultimum The pathogen causes ripe fruit rot of tomato Pearson et al. [33]
Pseudomonas chlororaphis
 HT66 PCN, wild-type, AmprSpr This study
 ∆phzI HT66 derivative, phzI deleted This study
 ∆phzR HT66 derivative, phzR deleted This study
 ∆csaI HT66 derivative, csaI deleted This study
 ∆hdtS HT66 derivative, hdtS deleted This study
Plasmids
 pK18mobsacB Broad-host-range gene replacement vector; sacB, Kmr Schafer et al. [47]
 pME6032 IPTG-inducible expression vector, Tcr Heeb et al. [48]
 pME-phzI phzI from HT66 in pME6032,Tcr This study
 pME-csaI csaI from HT66 in pME6032,Tcr This study
 pME-hdtS hdtS from HT66 in pME6032,Tcr This study
  1. r resistance