Table 1 Bacterial strains, and plasmids used in this study

Escherichia coli strains

 E.coli DH5α

supE44∆lacU169(ɸ80lacZ∆M15)recAhsdR17 recA1 endA1 gyrA96 thi-1 relA-1

Hanahan [45]

 E. coli S17

res^- pro mod⁺ integrated copy of RP4, mob⁺, used for incorporating constructs in P. chlororaphis

Hoffmann et al. [46]

 A. tumefaciens NTL4 (pZLR4)

Biosensor strain for AHLs

Cha et al. [28]

 C. violaceum CV026

Biosensor strain for AHLs

McClean et al. [29]

 Pythium ultimum

The pathogen causes ripe fruit rot of tomato

Pearson et al. [33]

Pseudomonas chlororaphis

 HT66

PCN, wild-type, Amp^rSp^r

This study

 ∆phzI

HT66 derivative, phzI deleted

This study

 ∆phzR

HT66 derivative, phzR deleted

This study

 ∆csaI

HT66 derivative, csaI deleted

This study

 ∆hdtS

HT66 derivative, hdtS deleted

This study

Plasmids

 pK18mobsacB

Broad-host-range gene replacement vector; sacB, Km^r

Schafer et al. [47]

 pME6032

IPTG-inducible expression vector, Tc^r

Heeb et al. [48]

 pME-phzI

phzI from HT66 in pME6032,Tc^r

This study

 pME-csaI

csaI from HT66 in pME6032,Tc^r

This study

 pME-hdtS

hdtS from HT66 in pME6032,Tc^r

This study