Fig. 4

BLI sensorgrams of kinetic analyses of CEACAM5 binding to immobilized common light chain IgG variants 4G07 (a) and 4F02 (b). Association with recombinant CEACAM5 at concentrations of 25, 12.5, 6.25 and 3.125 nM was measured for 400 s while dissociation was measured for 900 s. Fitting (red lines) of binding curves (colored lines) was calculated using a 1:1 binding model and Savitzky-Golay filtering