Fig. 3
From: A novel one-step approach for the construction of yeast surface display Fab antibody libraries
Screening of CEACAM5 common light chain YSD immune libraries by FACS. Yeast cells were consecutively incubated with 1 µM of His tagged CEACAM5 followed by secondary labeling with Alexa Fluor 647 conjugated anti-Penta-His antibody (target binding) and PE conjugated anti-kappa-antibody (display). Top: two-directional system; bottom: bicistronic system. In sorting round 3 (bicistronic system) the sorting gate was adjusted according to Additional file 1: Figure S2, since secondary reagent binders were detected