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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Chemical genomic guided engineering of gamma-valerolactone tolerant yeast

Fig. 4

Identifying genes mediating GVL toxicity by deletion and overexpression mutant profiling. The p-value of mutants with an increased fitness in the presence of 2.3% GVL compared to blank medium is plotted on the Y-axis (a n = 3). Of the top 20 significantly responsive mutants, strains annotated to the GO process Late Phenylpropanoid metabolic process (GO:0009698) are presented in green. GVL IC50 values of pad1Δ and fdc1Δ versus the control strain (b Mean ± SE, n = 3). Using the xylose fermenting yeast Y133 transformed with the 2 µ barcoded plasmid collection (MoBY 2.0), we identified genes that confer GVL sensitivity when overexpressed (c, n = 3). The p-value of overexpression mutants with a decreased fitness in the presence of 2.3% GVL compared to blank medium is plotted on the Y-axis, and the strain harboring pPAD1 is highlighted in red. GVL IC50 values of pPAD1 versus an empty vector (d, Mean ± SE, n = 3)

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