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Table 1 Cellulase A production and yield in different fermentation media over different phases (exponential growth phase versus early stationary phase and late stationary phase)

From: Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans

Medium

End of Exponential phase

Early stationary phase

Late stationary phase

CelA concentration

(mg/L)

Yield of CelA on biomass

(mg/gDCW)

Fermentation time (h)

CelA concentration

(mg/L)

Yield of CelA per unit of biomass

(mg/gDCW)

Fermentation time

(h)

CelA concentration

(mg/L)

Yield of CelA per unit of biomass

(mg/gDCW)

Fermentation time

(h)

MM

6.8

2.5

33

12.9

4.5

48

–

–

–

MMC5

12.3

2.7

28

22.0

5.5

44

–

–

–

MMC15

1.5

0.4

23.3

15.7

2.8

35

–

–

–

TSB

1.1

0.4

17

5.4

2.1

35

–

–

–

Bennet

4.9

1.5

65

4.9

2.2

67.2

–

–

–

NB

19.2*

22.4**

28

33.5*

37.7**

48

72.1

119.6

168

NB2X

27.5*

13.9**

23

60.8*

25.2**

42

–

–

–

  1. The italics numbers indicate the difference in CelA amounts produced in NB and NB2x media
  2. MM, minimal medium; C5 and C15, casamino acids (5 or 15 g/L); TSB, tryptic soy broth; NB, nutrient broth; NB2x, nutrient broth double strength; DCW, dry cell weight. n = 3
  3. * The changes in CelA amount in (mg/L). The amount significantly increases with doubling the amount of media
  4. ** The changes in CelA amount in (mg/ g DCW). The amount of CelA significantly decreases with doubling the amount of the media
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Microbial Cell Factories

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