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Table 1 Cellulase A production and yield in different fermentation media over different phases (exponential growth phase versus early stationary phase and late stationary phase)

From: Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans

Medium End of Exponential phase Early stationary phase Late stationary phase
CelA concentration
(mg/L)
Yield of CelA on biomass
(mg/gDCW)
Fermentation time (h) CelA concentration
(mg/L)
Yield of CelA per unit of biomass
(mg/gDCW)
Fermentation time
(h)
CelA concentration
(mg/L)
Yield of CelA per unit of biomass
(mg/gDCW)
Fermentation time
(h)
MM 6.8 2.5 33 12.9 4.5 48
MMC5 12.3 2.7 28 22.0 5.5 44
MMC15 1.5 0.4 23.3 15.7 2.8 35
TSB 1.1 0.4 17 5.4 2.1 35
Bennet 4.9 1.5 65 4.9 2.2 67.2
NB 19.2* 22.4** 28 33.5* 37.7** 48 72.1 119.6 168
NB2X 27.5* 13.9** 23 60.8* 25.2** 42
  1. The italics numbers indicate the difference in CelA amounts produced in NB and NB2x media
  2. MM, minimal medium; C5 and C15, casamino acids (5 or 15 g/L); TSB, tryptic soy broth; NB, nutrient broth; NB2x, nutrient broth double strength; DCW, dry cell weight. n = 3
  3. * The changes in CelA amount in (mg/L). The amount significantly increases with doubling the amount of media
  4. ** The changes in CelA amount in (mg/ g DCW). The amount of CelA significantly decreases with doubling the amount of the media