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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans

Fig. 3

Time course of S. lividans TK24 with pIJ486-vsi-celA and S. lividans TK24 with pIJ486 (as a wild type) growth in NB medium. a The growth curves of S. lividans TK24 carrying empty pIJ486 or pIJ486 expressing SPVsi-CelA in the nutrient broth expressed as values of dry cell weight. b Polypeptides (1.2–6 µg/lane) from culture supernatants (~ 7 µL/lane; equivalent to 0.08 mg of dry cell weight) from cells grown for the indicated times in NB were analyzed by SDS-PAGE and silver-stained. Lanes 1 and 12, MW markers as in Fig. 1b. Secreted CelA (filled arrow) is indicated

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Microbial Cell Factories

ISSN: 1475-2859

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