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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans

Fig. 2

Time course of CelA secretion and the effect of additional carbon source. a Polypeptides (0.1–5 µg/lane) from culture supernatants (0.9–16 µL/lane) that is equivalent to (0.08 mg of dry cell mass) from cells grown for the indicated times in the indicated media were analyzed by SDS-PAGE and silver-stained. Lane 1, MW markers as in Fig. 1b. Secreted CelA (filled arrow) is indicated. b The growth curves of S. lividans TK24 carrying pIJ486 expressing SPVsi-CelA in the indicated media expressed as values of dry cell weight. c The dry cell weight in gram per liter produced by S. lividans TK24 with pIJ486 expressing SPVsi-CelA in the different media (as in Fig. 1b) at the indicated growth phases. Ben, Bennet medium. d The amount of CelA secreted (mg) correlated to a gram of dry cell mass by S. lividans TK24 with pIJ486 expressing SPVsi-CelA in the different media (as in Fig. 1b) for the indicated time related to its growth curves in the same media. e, f The amount of CelA secreted (mg) correlated to a gram of dry cell mass (e) or 1L of culture (f) produced by S. lividans TK24 with pIJ486 expressing SPVsi-CelA in the different media at late exponential, early stationary and late stationary phases from d difference in CelA secretion was compared using an unpaired t test without assumption of equal variance (see “Methods”; NS, non-significant difference). Error bars represent standard error of the mean (SEM). n = 3

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