Fig. 4
From: A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
a Secretory expression of Ffu-fused target proteins in E. coli at time intervals of 0.5, 1, and 2 h. The conditions for expression of the proteins were: 0.5 mM IPTG at 25 °C. Key: soluble fractions (S) and inclusion bodies (ib). b The periplasmic analysis of Ffu-fused targeted proteins in E. coli. The conditions for expression of the proteins were: 0.5 mM IPTG at 25 °C for 1 h. Key: soluble fractions (S) and periplasmic fractions (P)