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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

Fig. 3

a Fluorescence distribution of S. cerevisiae cells after incubation with K28-mCherryHDEL. S. cerevisiae BY4742 was washed twice after 2 h incubation with K28-mCherryHDEL and analyzed by confocal laser scanning microscopy. b Same cells analysed by structured illumination microscopy (SIM). The hypersensitive yeast strain 192.2d was treated with K28-mCherryHDEL as described in a and subjected to SIM. (c) Fluorescence quenching was performed on cells of S. cerevisiae BY4742 after incubation with K28-mTFPHDEL as described in a, however extracellular mTFP fluorescence was subsequently quenched by the addition of 2 mM bromophenol blue (BPB)

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Microbial Cell Factories

ISSN: 1475-2859

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