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Table 1 The SPase expression from B. subtilis with pBSMuL3-SPase plasmid in flasks

From: Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme

Time(h)

12

24

36

48

OD600

6.5

7.4

8.5

8.3

CS SPase activity (U/mL)

0.8

1.4

1.4

1.6

WCL SPase activity (U/mL)

0.8

0.8

0.8

0.6

  1. CS culture supernatant, WCL whole-cell lysates