Fig. 4From: Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus Conversion of lactose into lactulose in batch reaction using parental (A) and Y114E enzymes (B). Each enzyme was incubated with 200 g/l of substrate solution at 65 °C for 4 h. Lactose (●), lactulose (○), epilactose (▼)Back to article page