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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus

Fig. 3

Effects of temperature and lactose concentration on activity using parental and Y114E enzymes. Lactulose (parental enzyme) (), lactulose (Y114E enzyme) (), epilactose (parental enzyme) () and epilactose (Y114E enzyme) (). A Effect of reaction temperature. Each 50 μg of enzyme was incubated with 20 g/l substrate solution at the range of 37–75 °C for 2 h. B Effect of lactose concentration. Each 50 μg of enzyme was incubated with 20–200 g/l substrate solution at 65 °C for 2 h

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