Fig. 3From: Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus Effects of temperature and lactose concentration on activity using parental and Y114E enzymes. Lactulose (parental enzyme) (●), lactulose (Y114E enzyme) (○), epilactose (parental enzyme) (▼) and epilactose (Y114E enzyme) (△). A Effect of reaction temperature. Each 50 μg of enzyme was incubated with 20 g/l substrate solution at the range of 37–75 °C for 2 h. B Effect of lactose concentration. Each 50 μg of enzyme was incubated with 20–200 g/l substrate solution at 65 °C for 2 hBack to article page