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Table 1 Plasmids and strains used in this study

From: Improving phloroglucinol tolerance and production in Escherichia coli by GroESL overexpression

Plasmids and strains Relevant characteristics References
Plasmids
 pET-28a(+) oripBR322 lacI q T7p, Kan r Novagen
 pET-phlDmarA pET30a carrying phlD from P. fluorescens Pf5, marA from E. coli DH5α, Kan r [7]
 pA-accADBC pACYCduet-1 carrying ACCase from E. coli DH5α, Cm r [7]
 pA-phlD/marA/acc pACYCDuet-1 carrying phlD from P. fluorescens Pf5, marA and ACCase from E. coli DH5α, Cm r This study
 pET28a-groESL pET-28a(+) carrying groESL from E. coli BL21(DE3), natural promoter, Kan r This study
 pET28a-tac-groESL pET-28a(+) carrying groESL from E. coli BL21(DE3), Tac promoter, Kan r This study
 pET28a-T7-groESL pET-28a(+) carrying groESL from E. coli BL21(DE3), T7 promoter, Kan r This study
Strains
 BL21(DE3) F omp T, hsdSB (r B m B ), gal, dcm me131, λ(DE3) Invitrogen
 G0 E. coli BL21(DE3)/pET-28a(+) This study
 G1 E. coli BL21(DE3)/pET28a-groESL This study
 G2 E. coli BL21(DE3)/pET28a-tac-groESL This study
 G3 E. coli BL21(DE3)/pET28a-T7-groESL This study
 PG0 E. coli BL21(DE3)/pA-phlD/marA/acc, pET-28a(+) This study
 PG1 E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-groESL This study
 PG2 E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-tac-groESL This study
 PG3 E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-T7-groESL This study