Table 1 Plasmids and strains used in this study

Plasmids

 pET-28a(+)

oripBR322 lacI ^q T7p, Kan ^r

Novagen

 pET-phlDmarA

pET30a carrying phlD from P. fluorescens Pf5, marA from E. coli DH5α, Kan ^r

[7]

 pA-accADBC

pACYCduet-1 carrying ACCase from E. coli DH5α, Cm ^r

[7]

 pA-phlD/marA/acc

pACYCDuet-1 carrying phlD from P. fluorescens Pf5, marA and ACCase from E. coli DH5α, Cm ^r

This study

 pET28a-groESL

pET-28a(+) carrying groESL from E. coli BL21(DE3), natural promoter, Kan ^r

This study

 pET28a-tac-groESL

pET-28a(+) carrying groESL from E. coli BL21(DE3), Tac promoter, Kan ^r

This study

 pET28a-T7-groESL

pET-28a(+) carrying groESL from E. coli BL21(DE3), T7 promoter, Kan ^r

This study

Strains

 BL21(DE3)

F⁻ omp T, hsdS_B (r ⁻_B m ⁻_B ), gal, dcm me131, λ(DE3)

Invitrogen

 G0

E. coli BL21(DE3)/pET-28a(+)

This study

 G1

E. coli BL21(DE3)/pET28a-groESL

This study

 G2

E. coli BL21(DE3)/pET28a-tac-groESL

This study

 G3

E. coli BL21(DE3)/pET28a-T7-groESL

This study

 PG0

E. coli BL21(DE3)/pA-phlD/marA/acc, pET-28a(+)

This study

 PG1

E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-groESL

This study

 PG2

E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-tac-groESL

This study

 PG3

E. coli BL21(DE3)/pA-phlD/marA/acc, pET28a-T7-groESL

This study