Skip to main content

Table 2 Description of sdAbs and variants

From: Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein

Clone name Description of mutations Charge Δ
EBOV-GP-G6 Original clone 0
EBOV-GP-G6-neg+ Disulfide addition: A54C, I78C − 1
FR1 changes: Q5V, A6E
EBOV-GP-H7 Original clone 0
EBOV-GP-H7-neg+ Disulfide addition: A54C, I78C − 1
FR1 changes: Q5V, A6E
EBOV-GP-A8 Original clone 0
EBOV-GP-A8-neg+ Disulfide addition: A54C, I78C − 2
FR1 changes: Q5V, A6E, A13V, G17D
EBOV-GP-A8-fneg+ Disulfide addition: A54C, I78C − 2
FR1 changes: Q5V, A6E, A13V, G17D
Unpaired Cys mutated: C106S
  1. Three of the GP binding sdAbs showing the highest melting temperatures and/or best affinities were subject to mutagenesis to improve their stability. Mutations included addition of a non-canonical disulfide bond, changes to FR1 that added negative charge, and “fixing” clone EBOV-GP-A8 by mutating an unpaired Cys