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Table 1 Binding affinity and melting temperature (Tm) for EBOV GP binding sdAb

From: Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein

Clone name

Tm (°C)

ka (1/Ms)

kd (1/s)

KD (M)

EBOV-GP-E7

54

3.5E+03

1.1E−04

3.4E−08

EBOV-GP-G6

67

7.0E+04

1.5E−04

2.0E−09

EBOV-GP-H7

68

1.7E+04

3.0E−04

1.7E−08

EBOV-GP-A8

60

3.1E+04

7.2E−05

2.3E−09

EBOV-GP-D1

51

3.0E+04

3.3E−04

1.1E−08

EBOV-GP-B5

60

3.4E+04

3.7E−04

1.1E−08

EBOV-GP-C12

62

3.28E+03

8.71E−05

2.68E−08

EBOV-GP-G11

46

No binding

No binding

No binding

EBOV-GP-G3

61

No binding

No binding

No binding

EBOV-GP-B11

62

8.98E+04

3.21E−03

3.59E−08

  1. Ten sdAbs representing the sequence families identified in selections for EBOV GP were characterized in terms of melting temperature and binding ability. Melting temperatures were determined by a fluorescent dye melt assay. Binding kinetics were measured by surface plasmon resonance (SPR)