Fig. 3

Secretion of Fm PEP in NZ9000. a Expression construct for secreted Fm PEP, USP: USP45, PP: propeptide, His6: HisTag, Term: terminator. b Growth curves for pNZ8148 vector only, USP45-FmPEP, USP45-PP1-, -PP2-, -PP3-, -PC-FmPEP. Induction is indicated by a red arrow. c Comparison of media fractions on a representative Western blot. Vector refers to expression of an empty pNZ8148 vector. d Representative Western blot comparison of soluble (S) and insoluble (IS) fractions in the cell lysate. Vector refers to expression of an empty pNZ8148 vector. Cell lysate and secreted fractions were concentrated 3 and 25 times respectively and 2 μL of each was loaded onto the gel. e % secretion yields and secretion efficiencies for the various constructs with respect to USP45-Fm PEP. Secretion protein yields are calculated based on densitometry. Enzyme activities were calculated based on Z-gly-pro-4-nitroanilide assay. Biological and technical triplicates were performed for these experiments, significant at *p < 0.05, **p < 0.01. Results are summarized in Additional file 1: Table S1