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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A propeptide toolbox for secretion optimization of Flavobacterium meningosepticum endopeptidase in Lactococcus lactis

Fig. 2

Secretion of TRX in NZ9000. a Expression construct for secreted TRX, USP: USP45, PP: propeptide, Linker: flexible linker with TEV cleavage site, His6: HisTag, Term: terminator. b Growth curves for pNZ8148 vector only, USP45-TRX, USP45-PP1-, -PP2-, -PP3-, -PC-TRX. Induction is indicated by a red arrow. c Representative Western blot of cell lysate (C) and secreted (S) fractions. Cell lysate and secreted fractions were concentrated 3 and 25 times respectively and 2 μL of each was loaded onto the gel. The lowest conserved band observed in the cell lysate fraction is a non-specific binding artefact (Additional file 1: Figure S1). % protein yields, with reference to USP45-TRX, and secretion efficiencies of the various TRX constructs are given below. These were calculated based on densitometry and technical triplicates

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Microbial Cell Factories

ISSN: 1475-2859

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