Table 3 Primers used in this study

3274

TATTCACGTAGACGGATAGGTATAGC

Amplification SHR I

3275

GTGCCTATTGATGATCTGGCGGAATG

Amplification SHR A

3276

GTTGAACATTCTTAGGCTGGTCGAATC

Amplification SHR B

3283

ACGTCTCACGGATCGTATATGC

Amplification SHR C

3597

ATTAAGGGTTCTCGAGAGC

Amplification natNT2

3750

GAGGCGTTAGTTTGGCTAATGAG

Diagnostic primer

3841

CACCTTTCGAGAGGACGATG

Amplification SHR B

3847

ACTATATGTGAAGGCATGGCTATGG

Amplification SHR A

3856

CTAGCGTGTCCTCGCATAGTTC

Amplification SHR C

4068

GCCTACGGTTCCCGAAGTATGC

Amplification SHR I

6005

GATCATTTATCTTTCACTGCGGAGAAG

pROS12 backbone

7389

GGTTTCTTAGACGTCAGGTGGC

pUC19 backbone

8076

GTTTAGCTCTATGGTGCAAAATTCTCCAGAAAAAAGGGATCCATAGAAAAGAATATGTCTAATTGAAAAATAGATATGTACCATAAGTAAAGTGCATGCGTGTATACCGAAACCAAGACA

Repair DNA SeURA3

8077

TGTCTTGGTTTCGGTATACACGCATGCACTTTACTTATGGTACATATCTATTTTTCAATTAGACATATTCTTTTCTATGGATCCCTTTTTTCTGGAGAATTTTGCACCATAGAGCTAAAC

Repair DNA SeURA3

8314

TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCTTGACTGATTTTTCCATGGAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAG

pROS12 + ScURA3 gRNA

8553

TGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAA

Repair DNA ScURA3

8554

TTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCA

Repair DNA ScURA3

9310

TCGCCTGCAAATCGTCATCG

Diagnostic primer ILV6

9311

CCTTAGAAACATCCGAGCTCCTCCTGGGCCTCTATACATC

Repair fragment construction

9312

GATGTATAGAGGCCCAGGAGGAGCTCGGATGTTTCTAAGG

Repair fragment construction

9313

AGCTGGTCGCCAAGGACTAC

Diagnostic primer ILV6

9314

CTACTGCGCCAATTGATGAC

Diagnostic primer ScURA3

9317

GCCCTACACGTTCGCTATGC

Diagnostic primer ScURA3

9318

GTTGACACAGTCCGTGAAAC

Diagnostic primer SeURA3

9321

GGCGCATTGGAGTCAATGAG

Diagnostic primer SeURA3

9390

ATGGATTATAAAGATGACGATGACAAAC

Amplification cas9

9391

CCGCTCAGACCTTTCTCTTC

Amplification cas9

9392

TTTTGTATAACTAAATAATATTGGAAACTAAATACG

Amplification ScPHO5t

9393

TGCCGAACTTTCCCTGTATGAAGCGATCTGACCAATCCTTTGCCGTAGTTTCAACGTATGTTTTCATTTTTGCGATGCCAG

Amplification ScPHO5t + addition SHR F

9394

TGTTGATTATGTTTTTAAGAACTACTCAGAATG

Amplification AaTEF1p

9395

AGGCCAGGAACCGTAAAAAG

pUC19 backbone

9396

ATTTCATTCTGAGTAGTTCTTAAAAACATAATCAACAATGGATTATAAAGATGACGATGACAAACCTCCAAAAA

LCR bridging oligo

9397

TGACCCTCCAAAAAAGAAGAGAAAGGTCTGAGCGGTTTTGTATAACTAAATAATATTGGAAACTAAATAC

LCR bridging oligo

9398

TTCATACAGGGAAAGTTCGGCAGGTTTCTTAGACGTCAGGTGGC

LCR bridging oligo

9399

CCTTTTTACGGTTCCTGGCCTCACCTTTCGAGAGGACGATG

LCR bridging oligo

9663

CATACGTTGAAACTACGGCAAAGGATTGGTCAGATCGCTTCATACAGGGAAAGTTCGGCATCAACATCTTTGGATAATATCAGAATGAG

Amplification panARSopt + addition of SHR F

10426

AATCTATAATCAGTCCATAGTCAACAAGAGCC

Amplification AaTEF1p

10427

TTTTCATTTTTGCGATGCCAGTTCTTTG

Amplification ScPHO5t

10432

AAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTGCGGCCGCGCTTCCAGGTTTGGCACTGTC

40 bp to pUC19 + NotI restriction + Left Homology arm fw

10433

ACTTTGAGGGCTCTTGTTGACTATGGACTGATTATAGATTTACGAAGGCACTTTGCATGGG

Left Homology arm rv + 40 bp to AaTEF1p

10434

GACAACACCTGTTGTAATCGAGCTCTCGAGAACCCTTAATGCGCGACATCAAATACCTTTGTCC

40 bp to natNT2 + right homology arm fw

10435

CACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCGCGGCCGCACGACGCGGACGAAGAGAAAG

Right homology arm rv + NotI restriction + 40 bp to pUC19

10436

ATAAGGGAAACTCAAAGAACTGGCATCGCAAAAATGAAAATAGGTCTAGAGATCTGTTTAGC

40 bp to ScPHO5t + natNT2 fw

10686

GAGTAAAGAAGCTCATCATTTATATAGATACGTTATGTAGATGTATAGAGGCCCAGGAGGGAGCTCGGATGTTTCTAAGGCTCTGTATGTACAAACTACGTATGTGACTTATACATTGCT

Repair DNA ILV6

10687

AGCAATGTATAAGTCACATACGTAGTTTGTACATACAGAGCCTTAGAAACATCCGAGCTCCCTCCTGGGCCTCTATACATCTACATAACGTATCTATATAAATGATGAGCTTCTTTACTC

Repair DNA ILV6

10992

GTTCAAGATGAATGTCTTGTCAAGATGATACAGAATGGGCATTCCCGGCGTATGGGATCTTCATGGCATCAAGCTTTTTCATTGGGTGTTTCTTCGACTAATGTGAAGGGAATGAACATT

Repair DNA SeATF1

10993

AATGTTCATTCCCTTCACATTAGTCGAAGAAACACCCAATGAAAAAGCTTGATGCCATGAAGATCCCATACGCCGGGAATGCCCATTCTGTATCATCTTGACAAGACATTCATCTTGAAC

Repair DNA SeATF1

10994

TTTGCTGTTTTGCGTAGGCAAAACATGTATTCGAATTTCGCTGTTTATGGGGAACTGAATAACGTTGGTGGTATGAACATGGACATGAGCGTAGTTCAGGGCACTCTACGGAATCGGGGC

Repair DNA SeATF2

10995

GCCCCGATTCCGTAGAGTGCCCTGAACTACGCTCATGTCCATGTTCATACCACCAACGTTATTCAGTTCCCCATAAACAGCGAAATTCGAATACATGTTTTGCCTACGCAAAACAGCAAA

Repair DNA SeATF2

10996

ATGAGAAAAATCAGGCCCCC

Diagnostic primer

10997

CTAAGGGCCTAAAAGGAGAGC

Diagnostic primer

10998

GAAGGATACGAACCACATATCACG

Diagnostic primer

10999

TAAAGCGACGCAAATTCGCC

Diagnostic primer

11000

CAGAAGAAAGCCAATTTAGCAG

Diagnostic primer

11001

TCAGGGATTTAAAAGCAGAGC

Diagnostic primer

11002

GGATAGTTTAGAGGAATACGAACCG

Diagnostic primer

11003

TATACGAGACCCGCGACG

Diagnostic primer