From: CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus
Name | Relevant genotype | Source | Addgene ID#a |
---|---|---|---|
pCT | ori amp r ARS4 CEN6 LEU2 AaTEF1p-Spcas9 D147Y P411T-ScPHO5 | [36] | Â |
pMEL15 | ori amp r 2 μm natNT2 SNR52p-gRNA CAN1 -SUP4t | [15] |  |
pROS12 | ori amp r 2 μm hphNT1 gRNA CAN1 gRNA ADE2 | [15] |  |
pUC19 | ori amp r LacZ multiple cloning site | [68] | Â |
pUD423 | ori amp r AaTEF1p-Spcas9 D147Y P411T-ScPHO5t | GeneArtâ„¢ | Â |
pUD526 | ori amp r NotI HRL AaTEF1p-Spcas9 D147Y P411T-ScPHO5t natNT2 HRMNotI | GeneArtâ„¢ | Â |
pUD527 | ori kan r SHRA AgTEF1p-amdS-AgTEF1t SHRB | GeneArtâ„¢ | Â |
pUD528 | ori kan r SHRB AaTEF1p-Spcas9 D147Y P411T-PHO5t | GeneArtâ„¢ | Â |
pUD530 | ori kan r SHRB panARSopt SHRC | GeneArtâ„¢ | Â |
pUD531 | ori kan r SHRC TDH3p BsaI BsaI CYC1t SHRI | GeneArtâ„¢ | Â |
pUD532 | ori kan r SHRI bla ori SHRA | GeneArtâ„¢ | Â |
pUD536 | ori amp r BsaI gRNA BsaISeILV6 | GeneArtâ„¢ | Â |
pUD573 | ori amp r BsaI gRNASeATF1 gRNA BsaISeATF2 | GeneArtâ„¢ | Â |
pUDP003 | ori amp r panARSopt AgTEF1p-amdS-AgTEF1t TDH3p BsaI BsaI CYC1t | This study | 101164 |
pUDP004 | ori amp r panARSopt AgTEF1p-amdS-AgTEF1t TDH3p BsaI BsaI CYC1t AaTEF1p-Spcas9 D147Y P411T-ScPHO5t | This study | 101165 |
pUDP010 | ori amp r panARSopt AgTEF1p-amdS-AgTEF1t TDH3p-HH-gRNA SeILV6 -HDV-CYC1t | This study | 101166 |
pUDP012 | ori amp r panARSopt AgTEF1p-amdS-AgTEF1t TDH3p-HH-gRNA SeILV6 -HDV-CYC1t AaTEF1p-Spcas9 D147Y P411T-ScPHO5t | This study | 101167 |
pUDP044 | ori amp r panARSopt AgTEF1p-amdS-AgTEF1t TDH3p-HH-gRNA SeATF1 -HDV-HH-gRNA SeATF2-HDV-CYC1t AaTEF1p-Spcas9 D147Y P411T-ScPHO5t | This study | 101168 |
pUDR107 | ori amp r 2 μm hphNT1 gRNA URA3 | This study |  |