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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

Fig. 4

Induction profiles with automated addition of 0–0.4 mM IPTG at different temperatures. Colors from blue to red indicate maximal FbFP intensities reached at the end of each culture. Black dots indicate the 35–42 individual cultivations for each temperature. The upper x-axis reflect the corresponding optical density of the cultures at the time of induction. It is calculated from the mean scattered light values of cultures that have not been induced until the respective induction time and a calibration curve that was previously prepared (see Additional file 1: Figure S5). Black boxes indicate conditions of parallel RAMOS cultivations. a 28 °C, induction after 4–11 h. b 30 °C, induction after 1–11 h. c 34 °C, induction after 1–10 h. d 37 °C, induction after 1–10 h. Cultivation conditions for E. coli Tuner(DE3)/pRhotHi-2-EcFbFP: 800 µL Wilms-MOPS mineral medium per well in a 48-Flowerplate sealed, with a sandwich membrane (m2p-labs), shaking frequency 1400 rpm and shaking diameter 3 mm

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