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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

Fig. 3

Comparison of selected induction conditions at 28 and 37 °C measured in a RAMOS and a BioLector device. Manual induction (shake flask) and automated induction (MTP) after 7 h with 0–0.4 mM IPTG (indicated by black arrows). Cultivation temperature was 28 °C for the graphs in the left column and 37 °C for the graphs in the right column. RAMOS and BioLector cultivations were conducted for each temperature in parallel using the same mastermix (medium plus microorganisms). a, b Oxygen-transfer rate of RAMOS cultivations in 250 mL shake flasks. c, d Scattered light signal from BioLector cultivations. e, f FbFP-fluorescence from BioLector cultivations. Cultivation conditions for E. coli Tuner(DE3)/pRhotHi-2-EcFbFP in RAMOS device: 8 mL Wilms-MOPS mineral medium in each 250 mL flask, shaking frequency 350 rpm, shaking diameter 50 mm, in BioLector device: 800 µL Wilms-MOPS mineral medium per well in a 48-Flowerplate, sealed with a sandwich membrane (m2p-labs), shaking frequency 1400 rpm and shaking diameter 3 mm

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