Fig. 2

Induction profile with automated addition of 0–1 mM IPTG solution after 1–10 h of cultivation time. Colors from blue to red indicate maximal FbFP intensities reached at the end of each culture. Black dots indicate the 36 individual cultivations. The upper x-axis reflects the corresponding optical density of the cultures at the time of induction. It is calculated from the mean scattered light values of the cultures that have not been induced until the respective induction time and a calibration curve that was previously prepared (see Additional file 1: Figure S5). Cultivation conditions for E. coli Tuner(DE3)/pRhotHi-2-EcFbFP: 800 µL Wilms-MOPS mineral medium per well in a 48-Flowerplate, sealed with a sandwich membrane (m2p-labs), 37 °C, shaking frequency 1400 rpm and shaking diameter 3 mm