Fig. 5

Involvement of TLR2 and CR3 in phagocytosis of the M. marinum strains by macrophages. Comparison of the M. marinum strains phagocytosis rates after blocking of macrophage TLR2 or CR3. Macrophages were pretreated with blocking antibodies (anti-TLR2 or anti-CR3) for 1 h and incubated with the M. marinum strains for 2 h at an MOI of 10. Non-ingested bacteria were extensively washed and killed by gentamycin. Macrophages were then lysed with Triton X-100 and the cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC for CFU determination. The data are presented as the mean number of ingested bacteria ± SEM from six separate experiments. For statistical significance: *p ≤ 0.03, (Mann–Whitney U test) the following datasets were compared: mutant/wild-type versus mutant/wild-type + blocking antibodies