Fig. 4

Impact of the M. marinum strains on TLR2 and CR3 expression. Macrophages were incubated with the various M. marinum strains for 2 h at an MOI of 10, after which the cells were stained with fluorochrome-conjugated antibodies for 30 min at 4 °C and analyzed by flow cytometry. The data are presented as the mean median fluorescence intensity (MFI) ± SEM from five separate experiments. For statistical significance *p ≤ 0.05 (Mann–Whitney U test) the following datasets were compared: uninfected macrophages versus macrophages infected with mutants or wild-type strain