Skip to main content

Table 2 Primers used in this study

From: Non-sterilized fermentation of high optically pure d-lactic acid by a genetically modified thermophilic Bacillus coagulans strain

Primers

Sequence (5′ → 3′)

L3-F

CCGGAATTCATGGAAATTTTTGATTATATGCG

L3-R

CCCAAGCTTTTACCGCGGAAGCCTTTTTTC

RTL3-F

TGAAAGAACCGAAGCACG

RTL3-R

CCGTTCTTCCGCCATCC

L1up-F

CCAGTACTCTGCAGAATTCGCTCCTTTCATTTGGTCAG

L1up-R

GTAAGCAAAAATTTTAGTCATATATAATCTTCCTCCCCATC

D-F

GATGGGGAGGAAGATTATATATGACTAAAATTTTTGCTTAC

D-R

GAAGCCCGGCCGGCACAAATGCTTAGCCAACCTTAACTGGAG

L1down-F

CTCCAGTTAAGGTTGGCTAAGCATTTGTGCCGGCCGGGCTTC

L1down-R

GCCGAAAATATGCACTCGAGGATCAACCGGGTCAGTGCAG

77-F

CTGCACTGACCCGGTTGATCCTCGAGTGCATATTTTCGGC

77-R

CTGACCAAATGAAAGGAGCGAATTCTGCAGAGTACTGG

pL1D-L1F

CGGGGTACCAGCCTCATCGCCGGTTTCC

pL1D-L1R

TAGGCAACAACTTTTCTCATATATAATCTTCCTCCCCATC

pL1D-DF

GATGGGGAGGAAGATTATATATGAGAAAAGTTGTTGCCTA

pL1D-DR

CCCAAGCTTTCATACTTTTATCTCCCACCTGCTC

pDD-F

CGGGGTACCGCATTCGTCTGAGTGGGCC

pDD-R

CCCAAGCTTTCATACTTTTATCTCCCACCTG

pL1LD-pL1F

GATTGTGAAATTGAATTCGAGCTCGGTACCAGCCTCATCGCCGGTTTCCCTCGC

pL1LD-LDR

GAAACAGCTATGACCATGATTACGCCAAGCTTTTAGCCAACCTTAACTGGAGTTTC

pDLD-pDF

CTGATTGTGAAATTGAATTCGAGCTCGGTACCGCATTCGTCTGAGTGGGCCAAGG

pDLD-pDR

GTAAGCAAAAATTTTAGTCATACGACAGCTTCCTTTCCATTC

pDLD-LDF

GAATGGAAAGGAAGCTGTCGTATGACTAAAATTTTTGCTTAC

pDLD-LDR

CTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGTTAGCCAACCTTAACTGGAG

  1. Restriction sites in the primer sequences are underlined