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Table 1 Strains and plasmids used in this study

From: Non-sterilized fermentation of high optically pure d-lactic acid by a genetically modified thermophilic Bacillus coagulans strain

Strains or plasmids

Features

Source

Bacillus coagulans

 DSM1

Wide type

DSMZ, Germany

 DSM1ΔldhL1ΔldhL2

ldhL1 and ldhL2 null mutant

This study

 d-DSM1

d-lactic acid producing strain

This study

 DSM1ΔldhL1ΔldhL2–pldhL1–BcldhD

B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhL1–BcldhD

This study

 DSM1ΔldhL1ΔldhL2–pldhD–BcldhD

B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhD–BcldhD

This study

 DSM1ΔldhL1ΔldhL2–pldhL1–LdldhD

B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhL1–LdldhD

This study

 DSM1ΔldhL1ΔldhL2–pldhD–LdldhD

B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhD–LdldhD

This study

 Lactococcus lactis MG1363

Host for gene cloning

Laboratory preservation

 Escherichia coli BL21(DE3)

Host for protein expression

Tiangen Co., China

 Escherichia coli Top10

Host for gene cloning

Tiangen Co., China

 pET-28a

Protein expression vector, KanR

Merck Co., Germany

 pGro7

Molecular chaperone, CmR

TaKaRa Co., Ltd, China

 pMH77

pSH71 replication containing temperature sensitive vector, CmR

Laboratory preservation

 pNW33n

E. coli-Bacillus shuttle vector, cloning vector, CmR

BGSC, USA

 pMH77–LdldhD

LdldhD insert vector, CmR

This study

 pNW33n–pldhL1–BcldhD

BcldhD expression vector with ldhL1 promoter, CmR

This study

 pNW33n–pldhD–BcldhD

BcldhD expression vector with BcldhD promoter, CmR

This study

 pNW33n–pldhL1–LdldhD

ldhL1 expression vector with LdldhD promoter, CmR

This study

 pNW33n–pldhD–LdldhD

ldhD expression vector with LdldhD promoter, CmR

This study

  1. Cm R chloramphenicol resistant, Kan R kanamycin resistant