Strains or plasmids | Features | Source |
---|---|---|
Bacillus coagulans | ||
DSM1 | Wide type | DSMZ, Germany |
DSM1ΔldhL1ΔldhL2 | ldhL1 and ldhL2 null mutant | This study |
d-DSM1 | d-lactic acid producing strain | This study |
DSM1ΔldhL1ΔldhL2–pldhL1–BcldhD | B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhL1–BcldhD | This study |
DSM1ΔldhL1ΔldhL2–pldhD–BcldhD | B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhD–BcldhD | This study |
DSM1ΔldhL1ΔldhL2–pldhL1–LdldhD | B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhL1–LdldhD | This study |
DSM1ΔldhL1ΔldhL2–pldhD–LdldhD | B. coagulans DSM1ΔldhL1ΔldhL2 with plasmid pNW33n–pldhD–LdldhD | This study |
Lactococcus lactis MG1363 | Host for gene cloning | Laboratory preservation |
Escherichia coli BL21(DE3) | Host for protein expression | Tiangen Co., China |
Escherichia coli Top10 | Host for gene cloning | Tiangen Co., China |
pET-28a | Protein expression vector, KanR | Merck Co., Germany |
pGro7 | Molecular chaperone, CmR | TaKaRa Co., Ltd, China |
pMH77 | pSH71 replication containing temperature sensitive vector, CmR | Laboratory preservation |
pNW33n | E. coli-Bacillus shuttle vector, cloning vector, CmR | BGSC, USA |
pMH77–LdldhD | LdldhD insert vector, CmR | This study |
pNW33n–pldhL1–BcldhD | BcldhD expression vector with ldhL1 promoter, CmR | This study |
pNW33n–pldhD–BcldhD | BcldhD expression vector with BcldhD promoter, CmR | This study |
pNW33n–pldhL1–LdldhD | ldhL1 expression vector with LdldhD promoter, CmR | This study |
pNW33n–pldhD–LdldhD | ldhD expression vector with LdldhD promoter, CmR | This study |