Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution

Table 1 Output fluxes observed by experiments (days 2–4) and the simulated output fluxes

Experimental or simulated data	Biomass	1,3-PDO	Glycerol	Sum of products 1,3-PDO + glycerol	1,3-PDO ratio 1,3-PDO/sum of products
TA2984 (parental strain for 1,3-PDO production)	0.0250	0.0237	0.0515	0.0752 (1.00)	0.315 (1.00)
Simulated data of NDH-1 elimination	0.0177	0.0852	0.0769	0.162 (2.15)	0.526 (1.67)
TA4021 (ndhD1-null strain of TA2984)	0.0292	0.0319	0.0835	0.115 (1.53)	0.277 (0.88)
TA4022 (ndhD2-null strain of TA2984)	0.0296	0.0299	0.0750	0.105 (1.40)	0.285 (0.90)
TA3672 (ndhD3-null strain of TA2984)	0.0290	0.0213	0.0562	0.0775 (1.03)	0.275 (0.87)
TA3671 (ndhD4-null strain of TA2984)	0.0371	0.0382	0.113	0.1512 (2.01)	0.253 (0.80)
TA4058 (ndhF1-null strain of TA2984)	0.0258	0.0497	0.163	0.213 (2.83)	0.233 (0.74)

ISSN: 1475-2859