Engineering Bacillus megaterium for production of functional intracellular materials

Table 1 Sporulation assays

Strain	Time at 85 °C (min)^c	From liquid culture		From solid medium		From solid medium
Strain	Time at 85 °C (min)^c	CFU/mL	Control (30 min 25 °C)	CFU/mL	Initial culture	CFU/mL	Initial culture
Positive control (WT spo)^a	30	1.6 × 10⁵		3.0 × 10⁶		2.1 × 10⁵
	45	2.2 × 10⁵	1.2 × 10⁷	7.1 × 10⁵	1.6 × 10⁶	2.3 × 10⁵	4.5 × 10⁶
	60	1.7 × 10⁵		9.6 × 10⁴		2.3 × 10⁵
ΔspoIIE	30	0		0		0
	45	0	2.4 × 10⁶	0	8.5 × 10⁶	0	1.6 × 10⁶
	60	0		0		0
Negative control (WT exp)^b	30	0		0		0
	45	0	3.2 × 10⁶	0	8.7 × 10⁵	0	2.1 × 10⁶
	60	0		0		0

^aPositive control: B. megaterium PHA05 wild type, sporulates on/in sporulation medium
^bNegative control: exponentially growing B. megaterium PHA05 wild type (in LB)
^c85 °C had been experimentally determined to be the optimum temperature for this assay (all vegetative cells killed, spores survive)

ISSN: 1475-2859