Fig. 3
From: Engineering Bacillus megaterium for production of functional intracellular materials
IgG binding assays using a purified IgG and b human serum. F, feed [IgG (a) or human serum (b)]; U unbound, E elution, CAB control beads not displaying ZZ domain, ZZ ZZ-domain displaying beads produced in this study, GE commercially available GE protein A beads; L, Mark 12â„¢ protein standard (Thermo Fisher Scientific). Beads were incubated with the respective feed material, washed repeatedly and IgG eluted with glycine pH 2.7. Asterisks indicate the IgG light and heavy chains