Fig. 2
From: Engineering Bacillus megaterium for production of functional intracellular materials
SDS-PAGE of ZZ-displaying and wild type beads recombinantly produced in B. megaterium PHA05 under control of the T7 promoter. Lane 1, ZZ-displaying beads, ZZ/ZZ-PhaC-ZZ protein at one asterisk (expected molecular weight 118 kDa); lane 2, wild type beads, PhaC protein at two asterisks (expected molecular weight 118 kDa). Equal amounts of protein as determined by Bradford were loaded in each lane. L, Mark 12™ protein standard (Thermo Fisher Scientific). The identity of the ZZ/ZZ-PhaC-ZZ fusion protein was confirmed by MALDI-TOF(MS)