Table 1 Plasmids used in this study
Plasmids | Description | Source |
|---|---|---|
pACYCDuet-1 | Expression vector, CmR, PT7, P15A ori | Novagen |
pCDFDuet-1 | Expression vector, SmR, PT7, CloDF13 ori | Novagen |
pRSFDuet-1 | Expression vector, KmR, PT7, RSF ori | Novagen |
pET28a | Expression vector, KmR, PT7, pBR322 ori | Novagen |
pET22b | Expression vector, AmpR, PT7, pBR322 ori | Novagen |
pETDuet-1 | Expression vector, AmpR, PT7, pBR322 ori | Novagen |
pCDF303 | Expression vector, SmR, PTrc, pCDF ori | |
pACYCDuet-1-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pACYCDuet-1 | This study |
pCDFDuet-1-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pCDFDuet-1 | This study |
pRSFDuet-1-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pRSFDuet-1 | This study |
pET28a-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pET28a | This study |
pET22b-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pET22b | This study |
pETDuet-1-SmP4H | Gene SmP4H inserted into NdeI/XhoI sites of pETDuet-1 | This study |
pACYC-dCas9 | Gene dCas9 inserted into NcoI/AvrII sites of pACYCDuet-1 | This study |
pCDF-anti-putA | anti-putA (high) sgRNA sequences inserted into EcoRI/BamHI sites of pCDF303 | This study |
pACYC-putP | Gene putP inserted into NcoI/XhoI sites of pACYCDuet-1 | This study |
pET28a-SmP4H-putP | Gene putP inserted into XhoI sites of pET28a-SmP4H | This study |
pET28a-LOGX | Gene LOGX inserted into NdeI/XhoI sites of pET28a | This study |