Fig. 6

The two strain coupling whole-cell system to supply α-KG from l-glutamate to produce CHOP. a Scheme of CHOP synthesis catalyzed by the co-cultures of recombinant E. coli BL21/pET28a-LGOX and E. coli BL21/pET28a-SmP4H-putP+anti-putA; b the variance of CHOP, l-proline, l-glutamate and α-KG under the condition of 5 g/L l-glutamate and 10 g/L l-proline; c the variance of CHOP, l-proline, l-glutamate and α-KG under the condition of 10 g/L l-glutamate and 10 g/L l-proline; d the variance of CHOP, l-proline, l-glutamate and α-KG under the condition of 20 g/L l-glutamate and 10 g/L l-proline. e The CHOP production with a fed-batch strategy under the condition of 20 g/L l-glutamate and 10 g/L l-proline