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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Process optimization for enhancing production of cis-4-hydroxy-l-proline by engineered Escherichia coli

Fig. 4

The effect of l-proline uptake on CHOP production. a The CHOP production by permeabilized cells of E. coli BL21/pET28a-SmP4H; b the effect of overexpressing putP in E. coli BL21/pET28a-SmP4H with different plasmids on CHOP production; c the effect of overexpressing putP in E. coli BL21/SmP4H with different plasmids on intracellular CHOP level. The relative CHOP abundance was calculated by normalizing the peak area of CHOP with internal standard. Results are expressed as mean ± standard error of the mean (n = 3). Significance levels of Students t test: *P < 0.01, **P < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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