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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Process optimization for enhancing production of cis-4-hydroxy-l-proline by engineered Escherichia coli

Fig. 3

Improved CHOP production by repressing endogenous l-proline degradation with CRISPRi technology. a PutA activity in crude extracts of BL21/pET28a-SmP4H and BL21/pET28a-SmP4H+anti-putA; b the effect of putA repression on l-proline degradation in the whole-cell of BL21/SmP4H. The reaction mixture contained resting cells (OD600 = 10), 200 mM PBS buffer (pH = 6.5), 10 g/L l-proline; c the effect of putA repression on CHOP production in the whole-cell of BL21/SmP4H. The reaction mixture contained resting cells (OD600 = 10), 200 mM PBS buffer (pH = 6.5), 10 g/L l-proline, 13 g/L α-KG, 5.0 mM Fe2+, and 1.7 mM l-ascorbate

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