Fig. 3

Diauxic growth of T. oleaginosus cells when cultured in dual-carbon media. a TOLN media containing 1.5 g/L glucose + 1.5 g/L resorcinol and b TOLN media containing 1.5 g/L Xylose + 1.5 g/L resorcinol. Growth (closed circles, solid lines) and substrate utilization (open markers, dashed lines) for sugars (black) and resorcinol (blue). The left axis represents OD₆₀₀ while the right axis represents percent substrate remaining in the media. Sugars are preferentially consumed, although resorcinol is partially consumed by hour 12 in both mixed carbon media. The cell density remains unchanged for 18 h after exhaustion, resulting in diauxic growth. The data are the mean and error bars are standard deviation of biological replicates (n = 3). Lines are used only for visual aid