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Table 4 Comparison of IL tolerance in the generated TALE evolved strains in the current study and previously reported tolerances from different studies with each respective tolerant biological system

From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution

Strain IL’s type Description/mechanism Culturing environment Concentration % (w/v) criteria References
E. coli K-12 MG1655fadD mutant [C4C1Im]Cl ALE (90 days long) LB broth 7.0 [11]
E. coli DH1 [C2C1Im]Cl Efflux pump encoded by eilA from Enterobacter lignolyticus Minimal media (M9) 5.8 [9]
JBEI-10101 (E. coli DH1) [C2C1Im]Cl Native eilA pump from Enterobacter lignolyticus Minimal media (M9) 5.5 [8]
Thermophilic communities [C2C1Im][OAc] Thermophilic enrichment Raw material with minimal media 6.0 [10]
JBEI-13314 (E. coli DH1 rcdA mutant) [C2C1Im][OAc] Mutation of transcriptional regulator encoded by rcdA LB broth 3.0 [12]
E. coli K-12 MG1655 mutants MG4.7, MG 3.10, and MG 4.5 [C2C1Im][OAc] TALE isolate Minimal media (M9) 8.5 This work
E. coli DH1 mutants DH 5.10 and 15.2 [C2C1Im][OAc] TALE isolate Minimal media (M9) 8.5 This work
  1. The high performing isolates from this work were three K-12 MG1655 mutants (MG 4.7, MG 3.10, and MG 4.5) and two DH1 mutants (DH 5.10 and DH 15.2)