From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution
Strain | ILâs type | Description/mechanism | Culturing environment | Concentration % (w/v) criteria | References |
---|---|---|---|---|---|
E. coli K-12 MG1655 âfadD mutant | [C4C1Im]Cl | ALE (90 days long) | LB broth | 7.0 | [11] |
E. coli DH1 | [C2C1Im]Cl | Efflux pump encoded by eilA from Enterobacter lignolyticus | Minimal media (M9) | 5.8 | [9] |
JBEI-10101 (E. coli DH1) | [C2C1Im]Cl | Native eilA pump from Enterobacter lignolyticus | Minimal media (M9) | 5.5 | [8] |
Thermophilic communities | [C2C1Im][OAc] | Thermophilic enrichment | Raw material with minimal media | 6.0 | [10] |
JBEI-13314 (E. coli DH1 rcdA mutant) | [C2C1Im][OAc] | Mutation of transcriptional regulator encoded by rcdA | LB broth | 3.0 | [12] |
E. coli K-12 MG1655 mutants MG4.7, MG 3.10, and MG 4.5 | [C2C1Im][OAc] | TALE isolate | Minimal media (M9) | 8.5 | This work |
E. coli DH1 mutants DH 5.10 and 15.2 | [C2C1Im][OAc] | TALE isolate | Minimal media (M9) | 8.5 | This work |