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Table 4 Comparison of IL tolerance in the generated TALE evolved strains in the current study and previously reported tolerances from different studies with each respective tolerant biological system

From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution

Strain

IL’s type

Description/mechanism

Culturing environment

Concentration % (w/v) criteria

References

E. coli K-12 MG1655 ∆fadD mutant

[C4C1Im]Cl

ALE (90 days long)

LB broth

7.0

[11]

E. coli DH1

[C2C1Im]Cl

Efflux pump encoded by eilA from Enterobacter lignolyticus

Minimal media (M9)

5.8

[9]

JBEI-10101 (E. coli DH1)

[C2C1Im]Cl

Native eilA pump from Enterobacter lignolyticus

Minimal media (M9)

5.5

[8]

Thermophilic communities

[C2C1Im][OAc]

Thermophilic enrichment

Raw material with minimal media

6.0

[10]

JBEI-13314 (E. coli DH1 rcdA mutant)

[C2C1Im][OAc]

Mutation of transcriptional regulator encoded by rcdA

LB broth

3.0

[12]

E. coli K-12 MG1655 mutants MG4.7, MG 3.10, and MG 4.5

[C2C1Im][OAc]

TALE isolate

Minimal media (M9)

8.5

This work

E. coli DH1 mutants DH 5.10 and 15.2

[C2C1Im][OAc]

TALE isolate

Minimal media (M9)

8.5

This work

  1. The high performing isolates from this work were three K-12 MG1655 mutants (MG 4.7, MG 3.10, and MG 4.5) and two DH1 mutants (DH 5.10 and DH 15.2)