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Table 3 Selected clones from each genetically identical or similar cluster were selected for testing for tolerance to [C2C1Im][OAc] and [C4C1Im]Cl ionic liquids

From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution

TALEs IL type Concentration (%) Gene set Aver. growth rate (h−1) Aver. final OD600 Aver. lag-time (h)
MG 4.7 [C4C1Im]Cl 5.4 MG-BM-3A 0.26 ± 0.024 0.87 ± 0.039 4.88 ± 0.04
MG 3.10 [C4C1Im]Cl 5.4 MG-BM-3D 0.31 ± 0.009 0.94 ± 0.04 10.85 ± 1.24
MG 4.5 [C4C1Im]Cl 5.4 MG-BM-3E 0.30 ± 0.015 0.84 ± 0.082 3.19 ± 1.17
MG 11.10 [C2C1Im][OAc] 4.6 MG-EM-1 0.23 ± 0.003 1.69 ± 0.004 1.55 ± 0.006
MG 12.7 [C2C1Im][OAc] 4.6 MG-EM-1 0.27 ± 0.005 1.1 ± 0.03 1.9 ± 0.015
MG 10.9 [C2C1Im][OAc] 4.6 MG-EM-1A 0.09 ± 0.003 0.74 ± 0.004 1.99 ± 0.006
DH1 5.3 [C4C1Im]Cl 4.6 DH-BM-3A 0.32 ± 0.005 0.96 ± 0.01 12.51 ± 0.075
DH1 5.10 [C4C1Im]Cl 4.6 DH-BM-2 0.58 ± 0.023 0.61 ± 0.12 8.8 ± 1.059
DH1 6.7 [C4C1Im]Cl 4.6 DH-BM-1 0.36 ± 0.033 0.99 ± 0.094 5.62 ± 0.154
DH1 7.5 [C4C1Im]Cl 4.6 DH-BM-1 0.25 ± 0.025 1.39 ± 0.014 11.49 ± 3.604
DH1 13.10 [C2C1Im][OAc] 4.2 DH-EM-2A 0.14 ± 0.013 0.52 ± 0.385 9.59 ± 0.66
DH1 14.2 [C2C1Im][OAc] 4.2 DH-EM-2B 0.2 ± 0.027 0.36 ± 0.304 4.91 ± 1.083
DH1 13.8 [C2C1Im][OAc] 4.2 DH-EM-1 0.23 ± 0.007 0.6 ± 0.085 8.57 ± 3.393
DH1 15.2 [C2C1Im][OAc] 4.2 DH-EM-3C 0.28 ± 0.057 0.57 ± 0.148 6.33 ± 0.259
DH1 16.7 [C2C1Im][OAc] 4.2 DH-EM-3A 0.31 ± 0.014 0.26 ± 0.172 5.34 ± 0.44
  1. Each of the TALE-derived isolates is presented with the corresponding IL-type and concentration in which it was originally evolved along with phenotypic characteristics of each of the selected isolates