Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution

Table 3 Selected clones from each genetically identical or similar cluster were selected for testing for tolerance to [C₂C₁Im][OAc] and [C₄C₁Im]Cl ionic liquids

TALEs	IL type	Concentration (%)	Gene set	Aver. growth rate (h⁻¹)	Aver. final OD₆₀₀	Aver. lag-time (h)
MG 4.7	[C₄C₁Im]Cl	5.4	MG-BM-3A	0.26 ± 0.024	0.87 ± 0.039	4.88 ± 0.04
MG 3.10	[C₄C₁Im]Cl	5.4	MG-BM-3D	0.31 ± 0.009	0.94 ± 0.04	10.85 ± 1.24
MG 4.5	[C₄C₁Im]Cl	5.4	MG-BM-3E	0.30 ± 0.015	0.84 ± 0.082	3.19 ± 1.17
MG 11.10	[C₂C₁Im][OAc]	4.6	MG-EM-1	0.23 ± 0.003	1.69 ± 0.004	1.55 ± 0.006
MG 12.7	[C₂C₁Im][OAc]	4.6	MG-EM-1	0.27 ± 0.005	1.1 ± 0.03	1.9 ± 0.015
MG 10.9	[C₂C₁Im][OAc]	4.6	MG-EM-1A	0.09 ± 0.003	0.74 ± 0.004	1.99 ± 0.006
DH1 5.3	[C₄C₁Im]Cl	4.6	DH-BM-3A	0.32 ± 0.005	0.96 ± 0.01	12.51 ± 0.075
DH1 5.10	[C₄C₁Im]Cl	4.6	DH-BM-2	0.58 ± 0.023	0.61 ± 0.12	8.8 ± 1.059
DH1 6.7	[C₄C₁Im]Cl	4.6	DH-BM-1	0.36 ± 0.033	0.99 ± 0.094	5.62 ± 0.154
DH1 7.5	[C₄C₁Im]Cl	4.6	DH-BM-1	0.25 ± 0.025	1.39 ± 0.014	11.49 ± 3.604
DH1 13.10	[C₂C₁Im][OAc]	4.2	DH-EM-2A	0.14 ± 0.013	0.52 ± 0.385	9.59 ± 0.66
DH1 14.2	[C₂C₁Im][OAc]	4.2	DH-EM-2B	0.2 ± 0.027	0.36 ± 0.304	4.91 ± 1.083
DH1 13.8	[C₂C₁Im][OAc]	4.2	DH-EM-1	0.23 ± 0.007	0.6 ± 0.085	8.57 ± 3.393
DH1 15.2	[C₂C₁Im][OAc]	4.2	DH-EM-3C	0.28 ± 0.057	0.57 ± 0.148	6.33 ± 0.259
DH1 16.7	[C₂C₁Im][OAc]	4.2	DH-EM-3A	0.31 ± 0.014	0.26 ± 0.172	5.34 ± 0.44

Each of the TALE-derived isolates is presented with the corresponding IL-type and concentration in which it was originally evolved along with phenotypic characteristics of each of the selected isolates

ISSN: 1475-2859