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Table 1 Growth phenotypes for E. coli K-12 MG1655 and DH1 evolved populations endpoints on ILs ([C4C1Im]Cl and [C2C1Im][OAc])

From: Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution

Ionic liquid Strains Replicate # Starting conc. % Initial growth rate (h−1)a Average end conc. % Final growth rate for end conc. (h−1)a Change in IL conc. (%) Total number of flasks
[C4C1Im]Cl MG1655 ALE #1 1.5 0.3 6.2 0.2 ± 0.02 4.7 62
MG1655 ALE #2 1.5 0.2 6.2 0.1 ± 0.03 4.7 67
MG1655 ALE #3 1.5 0.3 4.9 0.3 ± 0.12 3.4 63
MG1655 ALE #4 1.5 0.2 5.6 0.1 ± 0.05 4.1 84
     5.7 ± 0.6 0.2   
DH1 ALE #5 1.5 0.2 4.8 0.2 ± 0.05 3.3 61
DH1 ALE #6 1.5 0.2 5.6 0.3 ± 0.08 4.1 72
DH1 ALE #7 1.5 0.2 5.6 0.1 ± 0.01 4.1 79
DH1 ALE #8 1.5 0.2 4.2 0.2 ± 0.02 2.7 50
     5.0 ± 0.6 0.2   
[C2C1Im][OAc] MG1655 ALE #9 2 0.2 5.9 0.1 ± 0.00 3.9 86
MG1655 ALE #10 2 0.2 5.9 0.1 ± 0.04 3.9 87
MG1655 ALE #11 2 0.2 6.5 0.2 ± 0.00 4.5 91
MG1655 ALE #12 2 0.2 5.9 0.1 ± 0.01 3.9 92
     6.1 ± 0.3 0.1   
DH1 ALE #13 1 0.1 4.5 0.1 ± 0.06 3.5 77
DH1 ALE #14 1 0.1 4.5 0.1 ± 0.02 3.5 92
DH1 ALE #15 1 0.2 4.5 0.2 ± 0.01 3.5 79
DH1 ALE #16 1 0.2 5.2 0.2 ± 0.02 4.2 88
     4.6 ± 0.3 0.2   
  1. aInitial and final growth rates were calculated for the whole population from the first and last 3 flasks of each corresponding population endpoints, respectively